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Tri-Sep™-2100 pCEC system
Features of TriSep™-2100 pCEC system
- Ultra Performance in Separation
Ultra Efficiency: Over 250,000 plates/m, 4 times better than HPLC
Ultra Speed: 5.5 mm/s of linear velocity, 5 times faster than HPLC
Ultra Peak Capacity: 3 times better than HPLC
Ultra Reproducibility: Excellent reproducibility like HPLC, and much better than CE
- All in One
Modular design and flexible combination make it possible to run CE, micro-HPLC and pCEC on the same TriSep™-2100 system!
- Advanced Mechanism
User of TriSep™-2100 will readily enjoy the leisure and excitement in research brought by the system features of high efficiency, high selectivity, high resolution and fast speed.
- Method Development
The direct transplanting of method from classic HPLC separation condition greatly shortens the time needed for method development.
- Gradient Eluting CEC
Elaborate micro-fluid control module dramatically decreases system dead-volume. High-pressure gradient eluting CEC mode dramatically improves the separation power.
- Dedicated Software
User-friendly TriSep™ Workstation software, which fully complies with GLP regulations, facilitates powerful data analysis!
Overview of TriSep™-2100 pCEC system
Pressurized capillary electrochromatography (pCEC) is a new revolutionary separation technology which achieves ultra performance. In pCEC, the retention mechanism is based on both chromatographic partition and electrophoresis. Thanks to the contribution of the dual mechanisms, pCEC successfully makes use of columns with small particles of 1.5µm, and dramatically enhances the efficiency, speed, peak capacity, reproducibility and sensitivity, compared to traditional HPLC and CE. Combined with on-column detection techniques, pCEC could be readily coupled with UV, LIF, and MS. Multi-mechanism makes CEC an ideal technique for the analysis of complicated samples in biological and chemical fields.
Currently, CEC technology is widely applied in various fields, including Pharmaceutical, Life Science, Toxicology, Forensic Medicine, Health Products, Chemical, Petrochemical, Environment, Chiral Compound, Food/Beverage, etc.
As the leader in nano and micro separations, Unimicro Technologies presented the world’s first dedicated pCEC system TriSep™-2000 and then developed the second generation pCEC system, TriSep™-2010GV. In 2004, Unimicro Technologies came up with the latest version, TriSep™-2100. Revolutionary design and engineering dramatically elevate the system performance, compared to the former models. Powerful technical support on application, brand-new coupling interface with various up-to-date detection technologies and patented EletroPak™ capillary columns make TriSep™-2100 an ideal tool for explorers in all research fields mentioned above.
Main Specifications of TriSep™-2100 pCEC system
Solvent Delivery System
Dual reciprocating plunger solvent delivery module, high gradient accuracy and ultra-low flow pulsation, automatic plunger seal rinsing.
Item |
Specification |
Pump type |
Micro- volume double plunger pump (approx. 10 m L per stroke ) |
Pumping methods |
Constant flow delivery and constant pressure delivery |
Constant flow pumping |
Flow rate setting range |
0.001-5mL/min (1.0-39.2MPa), 5.001-9.999mL/min(1.0-19.6MPa) |
Flow rate accuracy |
± 2% or ± 2 m L/min, whichever is greater(when water is pumped at 0.01-5mL/min, 1.0-39.2MPa, with room temperature 20 °C ) |
Flow rate precision |
Within ± 0.3% (when water is pumped at 0.1-5mL/min, 1.0-39.2MPa, with room temperature 20 °C ) |
Constant pressure pumping |
Pressure setting range |
1.0-39.2MPa [0.1MPa steps] |
Pressure accuracy |
± 10% or1.0MPa, whichever is greater |
Pressure limits |
Upper and lower limits |
Time program |
Commands for flow rate, pressure, EVENT functions, LOOP (for program repetition) 10files, 320 steps. |
Pressure display accuracy |
± 2% or 0.5MPa, whichever is greater |
Plunger cleaning line |
Automatic rinsing possible with optional kit |
Size |
W260 × H 140 × D 420mm , excluding projections |
Weight |
11kg |
Operating temperature range |
4-35 °C |
High-pressure Gradient System Specifications
Item |
Specification |
Number of solvents mixed |
2 |
Gradient profile |
Step and linear possible at multiple levels |
Maximum program steps |
Max.320 steps per program. 20program files |
Program duration |
Max.999.99 minutes (in 0.01minute steps) |
Mix ratio setting range |
0-100% (in 0.1% steps) |
Concentration accuracy |
± 1% (with aqueous acetone/water, at 0-100%, 0.1-3mL/min,1.0-39.2MPa) |
Flow rates poss inlet |
0.001-9.999mL/min |
Nano Injection Valve:
Optional sample loop volumes: 10 nl, 20 nl, 50 nl
UV/Vis Detector
Item |
TriSep ™ —2100 detector |
Light source |
Deuterium lamp |
Wavelength range |
190-600nm Cut-off filter to eliminate second order diffraction is automatically activated for the 371—600nm range. |
Spectral bandwidth |
8nm |
Wavelength accuracy |
1nm |
Wavelength reproducibility |
0.1nm |
Drift |
5 ×10 -4 AU/hour Max. |
Noise Level |
6 × 10 -6 AU |
Dual wavelength mode |
Measurement Wavelength |
Selectable, two wavelengths in 190—370nm or 371—600nm range |
Sampling frequency |
1.2 sec for one wavelength |
Response |
Selectable in 10 steps corresponding to time constant 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 seconds |
Range |
Can be set between 0.0001 and 2.56 AUFS in 0.0001 AUFS steps |
Zero adjustment |
Auto zero function, baseline shift function |
Polarity switching Wavelength scanning function |
Possible Performs wavelength scanning with flow stopped. Data stored in three files, one of which stores background. Background is subtracted from sample scans. File data is not saved up (lost when power is turned OFF). |
Wavelength steps |
1-5nm, selectable in 5 steps, |
Scanning speed |
2-5nm when W lamp is used. 10-50nm/sec, settable in 5 steps (according to the wavelength step) |
Spectrum plot output speed |
1, 3, 10 nm/sec |
Ratio chromatogram |
Outputs the absorption ratio of two wavelengths |
Time Program |
Available in detector or by system controller |
|
Set items |
Wavelength (including dual wavelengths), auto zero, range, marker, response, wavelength scanning, event, polarity, lamp ON/OFF, loop |
Number of steps (detector program) |
Maximum 32 steps |
Output |
Output for recorder |
10mV recorder terminals |
Output for integrator |
6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 AU/V Integrator output terminals output chromatogram in single wavelength mode or the Ch1 chromatogram in dual wavelength mode. Ratio chromatogram, spectrum, and Ch2 chromatogram are output from the recorder output terminals. |
Lamp hour-meter |
Records up to 9999.9 hours |
Size |
260(W) 140(H) 430(D) mm |
Weight |
13 kg |
Operating temperature range |
4°C — 35°C |
Power supply |
110-120V AC, 10 % 150VA 50-60Hz
220-230V AC/240V AC, 10 % 150VA 50-60Hz |
High Voltage power Supply
Output voltage |
0 ~ ± 30 kV |
Output current: |
0 ~ 100 µA |
Max Output power |
4 W |
Net weight |
12 kg |
LIF Detector (Optional)
Relative Fluorescence Units (RFU) range |
0 to 1000 RFU |
Dynamic Range (at RFU range 1000) |
10 6 |
Sensitivity |
1×10 -11 M with S/N>2 |
Noise |
<0.005 RFU |
Drift |
<0.01 RFU |
Wavelength Range (for optics) |
Excitation |
300 to 700 nm |
Emission |
350 to 750 nm |
Software compatibility |
TriSep-LIF software |
Laser |
10 mW 473 nm DPSS Laser |
Power Requirements |
AC110/220 V, 50/60 Hz |
Temperature Operating Range |
15-35 ° C |
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