MicroSolv Technology Corporation

Click here to view
catalog description

CelerityCE™ Application Sheet

Pyrimidine/Purine Bases

Short Analysis Time
Simple, Easy, Fast
Cholesterol Bonded Capillary

Method Conditions:
Voltage: 25kV
Capillary: CelerityCE™ Cholesterol Bonded Capillary, 50µm x 70cm
Injection: 5 seconds @ 5lcHg, Hydrodynamic
Length to Detector Window: 45cm
Run Buffer: 19mM Tris/30mM Phosphoric Acid
pH: 2.14
Detection: UV @260nm

PEAKS:

Thymine Hydrochloride
Cytosine
Adenine
Guanine Hydrochloride
Adenosine

Discussion of ElectroChromatogram:
All of these compounds can exist together in the same samples and are very important physiologically. They can be easily resolved using the CelerityCE™ Cholesterol Bonded Capillary. Note the highly symmetric peak shape. The high speed and resolution of this separation cannot be achieved by HPLC.

[TOP]