Protein Mixture on C18

• Short Analysis Time
• Simple, Easy, Fast
• C18 Bonded Capillaries with High Surface Area

Method:
A solution of Proteins was prepared and injected onto a CelerityCE™ C18 High Surface Area Capillary using the ABI 270A-HT CE system. A voltage of 30kV was applied across the capillary resulting in 15µA. The mobile phase 30mM Acetic Acid/ 37.5mM Gamma Amino Butyric Acid was prepared using Good Laboratory Practice. The Capillary was conditioned following the procedure suggested by the capillary manufacturer. Injection was Hydrodynamic for 5 seconds under 12.5cm of Hg

PEAKS: 1.Lysozyme (turkey) 2.Cytochrome-C 3.Ribonuclease A 4.Myoglobin 5.Unknown Impurity

Discussion and Rationale:
These proteins are series of compounds that are well separated during a short analysis time. Basic compounds that are as hydrophobic as these compounds are usually require a more hydrophilic phase to be separated with such high efficiency and high symmetry. These proteins are usually problematic for uncoated capillaries.

UV detection of 211nm was selected and sharp, well resolved peaks result. Each of these proteins are separated isocratically.