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MicroSolv Digested Protein
Calibration Standards and Kits
For MASS SPEC, CE or HPLC
Instead of spending your valuable time preparing and characterizing your own standards, why not use... MicroSolv
Digested Protein Calibration Standards and Kits
Tryptic Digest Standards for CE-MS, GPC, MALDI-TOF and LCMS Applications
- Save Time, Save Money
- Available as High or Low Molecular Weight Kits
- Lyophilized for long shelf life
- Characterized by HPLC and Maldi-TOF
Using standards for identification and calibration of separation methods is a cornerstone of
State of the Art Protein Chemistry. The MicroSolv Digested Protein Calibration Kits are
particularly well suited for method development and validation in most protein or Proteomic applications.
Useful for cIEF or CE-MS Applications, they are also very useful for LCMS, MALDI-TOF or GPC. Easy
to use and with long shelf life, these standards are available individually, in six packs or in convenient kits
for low or high molecular weights.
Complete with instructions on how to dilute and store each standard is lyophilized and supplied in a vial which
contains 500 pico-moles of concentrated protein digest that can be easily diluted to standard concentration.
Unopened standards have a long shelf life.
Two different, convenient kits are available for calibrating either high or low molecular weight peptide
applications or can be used for calibrating instruments.
- The Low Molecular Weight Kit contains six different vials each containing a different standard ranging
from 12 kilo Daltons to 40 kilo Daltons.
- The High Molecular Weight Kit contains six different vials each containing a different standard ranging
from 45 kilo Daltons to 160 kilo Daltons.
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| Molecular Weight Kits |
| Catalog # |
Description |
| 34922-9931 |
MicroSolv Low Molecular Weight Kit for Protein Calibration.
Contains 500picomoles of the each of the following: CytochromeC, Lysozyme, Hemoglobin,
Myoglobin, Carbonic Anhydrase, Conalbumin. |
| 31922-9932 |
MicroSolv High Molecular Weight Kit for Protein Calibration.
Contains 500picomoles of the each of the following: Peroxidase, Alpha amylase, Bovein Serum
Albumin, Catalase Lactic Dehydrongenase, Immuno Gamma Globulin (Igg). |
| Individual Standards Sold individually |
| Catalog # |
Description |
Source |
kDa |
| 34922-01 |
CytochromeC |
Equine Heart |
12 |
| 34922-02 |
Lysozyme |
Chicken Egg White |
14 |
| 34922-03 |
Hemoglobin |
Bovine Blood |
17 |
| 34922-04 |
Myoglobin |
Equine Skeletal Muscle |
17 |
| 34922-05 |
Carbonic Anhydrase |
Bovine Erythrocytes |
29 |
| 34922-06 |
Conalbumin |
Chicken Egg White |
40 |
| 34922-07 |
Peroxidase |
Horseradish |
44 |
| 34922-08 |
Alpha amylase |
Bacillus sp. |
50 |
| 34922-09 |
Bovine Serum Albumin (BSA) |
Bovine Serum |
68 |
| 34922-10 |
Catalase |
Bovine Liver |
128 |
| 34922-11 |
Lactic dehydrogenase |
Rabbit Muscle |
140 |
| 34922-12 |
ImmumoGammaGlobulin (Igg) |
Porcine Serum |
160 |
| Individual Standards Sold in Packs of Six |
| Catalog # |
Description |
Source |
kDa |
| 34922-6601 |
CytochromeC |
Equine Heart |
12 |
| 34922-6602 |
Lysozyme |
Chicken Egg White |
14 |
| 34922-6603 |
Hemoglobin |
Bovine Blood |
17 |
| 34922-6604 |
Myoglobin |
Equine Skeletal Muscle |
17 |
| 34922-6605 |
Carbonic Anhydrase |
Bovine Erythrocytes |
29 |
| 34922-6606 |
Conalbumin |
Chicken Egg White |
40 |
| 34922-6607 |
Peroxidase |
Horseradish |
44 |
| 34922-6608 |
Alpha amylase |
Bacillus sp. |
50 |
| 34922-6609 |
Bovine Serum Albumin (BSA) |
Bovine Serum |
68 |
| 34922-6610 |
Catalase |
Bovine Liver |
128 |
| 34922-6611 |
Lactic dehydrogenase |
Rabbit Muscle |
140 |
| 34922-6612 |
ImmumoGammaGlobulin (Igg) |
Porcine Serum |
160 |
Production Background of the MicroSolv Protein Calibration Kits
We begin with the highest purity proteins that are reduced with dithiothreitol (DTT) and alkylated with
iodoacetic acid (IAA) prior to Digestion. Using trypsin that is treated with L-(tosylamido-2-phenyl)ethyl
chloromethyl ketone (TPCK) allows us to inhibit unwanted, contaminating chymotryptic activity. Monitoring the
process by HPLC and Maldi-TOF (actual fragmentation data is supplied with each standard vial) also provides a
characterization of each individual standard.
The chemical, physical and toxicological properties of these products have not been thoroughly tested. Vials are
individually packed to contain 500 picomoles which should be stored at <0°C.
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