ANGIOTENSIN PEPTIDES

• Short Analysis Time
• Simple, Easy, Fast

Method:
A solution of Angiotensin I, II and III was prepared and injected onto a CelerityCE™ Diol High Surface Area Capillary using an Applied Biosystems 270A-HT Capillary Electrophoresis System. A voltage of 30kV was applied across the column resulting in 29µA. The mobile phase (19mM Tris/30mM Sodium Phosphate) was prepared using Good Laboratory Practice. The Column was conditioned following the procedure suggested by the column manufacturer. Injection was Hydrodynamic for 5 seconds under 12.5cm of Hg.

Discussion and Rationale:
Angiotensins are series of hydrophobic peptides that are involved in the regulation of blood pressure. Basic compounds that are as hydrophobic as these compounds are, usually require a more hydrophilic phase to be separated without tailing effects. Notice that in the separation of this mixture not only were high efficiencies achieved but also tailing is minimal. The analysis time is much quicker on a hydrophilic phase such as the Diol then on a hydrophobic phase (i.e. C18).

UV detection of 211nm was selected and sharp, well resolved peaks result. Each of these Angiotensins peptides are very similar in structure and size yet they are very well resolved.