CYTOCHROME C
Horse, Bovine, Chicken, Tuna

• Short Analysis Time
• Simple, Easy, Fast

Method:
A solution of horse, bovine, chicken and tuna Cytochrome-C was prepared and injected onto a CelerityCE™ C-18 High Surface Area Capillary using an a voltage of 30kV was applied across the capillary resulting in 15µA. The mobile phase (run buffer) was prepared using Good Laboratory Practice. The Capillary was conditioned following the procedure suggested by the capillary manufacturer. Injection was Hydrodynamic for 5 seconds under 12.5cm of Hg. Applied Biosystems 270A-HT Capillary Electrophoresis System.

Discussion and Rationale:
The separation of Cytochrome-C of various origins is easily and quickly accomplished using an Isocratic CEC method. All of these proteins are basic compounds which will interact with silanol sites and since the peaks in this chromatogram are all very symmetrical it indicates no strong interactions with the residual silanol sites. The excellent resolution indicates good efficiency and theoretical plates.

UV detection of 211nm was selected and sharp, well resolved peaks result. Each of these Cytochrome C proteins are very similar in structure and size yet they are very well resolved. Small peaks are due to impurities in the injected sample.