HPLC Application Sheet
CARVONE - Cogent UDC-Cholesterol™ Retention Map
4 Different Mobile Phases
Method Conditions
| Column |
Cogent UDC™ Cholesterol |
| Catalog No. |
69069-15R |
| Dimensions |
150mm x 4.6mm ID |
| Mobile Phase |
— Water/THF
— Water/Acetonitrile
— Water/Methanol
— Hexane/Isopropanol
|
| Flow Rate |
1mL/minute |
| Sample |
Carvone |
| Synonym |
2-cyclohexen-1-one, 2-methyl-5-(1-methylethenyl)methyl-5-(1-methylethenyl) |
| Injection Volume |
10µL |
| Detection |
255nm |
Discussion of Chromatogram:
The retention characteristics of Carvone on this UDC-Cholesterol Column in aqueous reverse phase mode is typical of
a C18 or C8 column. The retention times change respectively with THF being a stronger solvent than Acetonitrile
which is stronger than Methanol (shown).
This column can also be used in either Aqueous-normal phase or Organic-normal phase (shown above). Other phases such
as Cyano or Amino are not optimal choices if you want to switch between Aqueous-reverse phase and Organic normal
phase. UDC-Cholesterol and other Cogent TYPE-C HPLC Columns are unique as they require relatively minimal
re-equilibration effort to switch between Aqueous-normal phase and Reverse phase and back to Organic-normal phase.
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