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QUICK TOUR:

Understanding the Fundamentals of TYPE-C™ Silica-based HPLC Columns

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Introduction Page
Introduction Page
Definition of Normal and Reverse Phase
Overview of Presentation
Comparison of Evolutionary TYPE-C Silica to Current Silica
Comparison of Evolutionary TYPE-C Silica
to Current Silica

Silicon-Hydrides Dominate the surface
Non Polar Surface
Unique Bonding Capabilities
Unique Bonding Capabilities
Liquid Crystal and Bidentate Ligands
Silicon-Carbon Bonding Of Ligands
Silicon-Carbon Bonding Of Ligands
UDC-Cholesterol Liquid Crystal Phase
Organo-Silane Free
Retention Map Neutrals
Retention Map Neutrals
See how this Column Performs with Neutral Compounds
What is a Retention Map?
Retention Map Acids
Retention Map Acids
See how this Column Performs with Acidic Compounds
What is a Retention Map?
Retention Map Bases
Retention Map Bases
See how this Column Performs with Basic Compounds
What is a Retention Map?
Normal Phase Resolution of Basic Compounds
Normal Phase Resolution
of Basic Compounds

Use Aqueous Normal Phase or Organic with this Column
Predicting Elution Behavior of Common Compounds
Predicting Elution Behavior
of Common Compounds

Unexpected results even for experienced chromatographers
Choose a Different Separation Mechanism on the Same Column
Choose a Different Separation Mechanism on the Same Column
ARP or ANP or ONP
Useful for Complex Mixtures
Improved Organic Normal Phase Column
Improved Organic Normal Phase Column
Sharp Peaks, No Hysteresis
Real Life Application
Real Life Application
Metformin Glyburide Background
Real Life Application
Real Life Application
Isocratic Separation of Two Dissimilar Compounds
Changing Elution Order, Easily




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