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Hydrophobic and Silanophilic Interaction Tests
for Cogent TYPE-C Silicon-Hydride HPLC Columns
The “Neue Test”
Actual Chromatograms: Silanophilic Test
Cogent UDC-Cholesterol
4µm, 4.6 x7.5mm
Catalog No.: 69069-7.5P
Method Conditions:
Mobile Phase: 35:65, 20mM phosphate buffer, pH 7/Methanol
Flow Rate: 0.5ml/min
Injection Volume: 1µl
Detection: UV 214nm
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Dissolved in Methanol
Amitriptyline Hydrochloride 100mg/L
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Assymmetry Parameter (As): 0.950
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