MicroSolv Technology Corporation

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Hydrophobic and Silanophilic Interaction Tests
for Cogent™ TYPE-C™ Silicon-Hydride
HPLC Columns
The “Neue Test”

Actual Chromatograms: Hydrophobicity Test
Cogent Bidentate C18™
4µm, 4.6 x7.5mm
Catalog No.: 40018-75P

Method Conditions:
Mobile Phase: 35:65, 20mM phosphate buffer, pH 7/Methanol
Flow Rate: 0.5ml/min
Injection Volume: 1µl
Detection: UV 214nm

Sample:

Dissolved in Methanol
Uracil 16mg/L
Napthalene 60mg/L
Acenaphthene 200mg/L

%RSD for Acenaphthene: 0.25%

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