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About TYPE-C Silica™ based HPLC products?
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Hydration Shell, Liquid Stationary Phase type-B v. TYPE-C™
Strong adsorption of water to type-B silica (shown above) makes using normal phase chromatography very difficult.
The silicon-hydride groups (Si-H) found on the surface of TYPE-C Silica™ are not prone to such strong water
retention (shown above) as type-B silica making it an excellent choice for Organic-Normal Phase with improvements
in speed and range of solvent possibilities. The weaker water adsorption also accounts for the little to no
hysteresis observed when changing from Organic-Normal to Aqueous-Normal/Reverse Phase with TYPE-C™ products or
when changing pH with TYPE-C Silica™ based HPLC columns such as Cogent UDC-Cholesterol™. This feature
makes the column preferred over polar-embedded phases which often exhibit long term memory effects. TYPE-C
Silica™ products extend the useful range of Normal phase from Hexane/Ethyl Acetate all the way to
Water/Acetonitrile with excellent precision.
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