|
|
Reproducibility:
Cogent Silica-C™ Columns
The Chromatogram shown here is actually six chromatograms laid over each other. This clearly shows how reproducible the
method and column are from run to run.
Method Conditions
| Column |
Cogent Silica-C™, 4µm, 100A |
| Catalog No. |
40000-75P |
| Dimensions |
4.6 x 75mm |
| Solvents |
A: DI water + 0.1 % formic acid
B: acetonitrile + 0.1% formic acid |
| Peaks |
1. L-(+)-alpha-phenylglycine
2. L-phenylalanine |
| Mobile Phase |
80%B/20%A
|
| Flow rate |
1.0 mL/minute (t0 = 0.85 min) |
| Injection Volume |
2 µL |
| Sample Matrix |
0.3 mg/mL of each sample dissolved in 50% acetonitrile/50% DI water + 0.5% formic acid. |
| Detection |
254 nm UV |
|
