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HPLC Application
Acidic Compounds

Acids Test:
Mobile Phase:	35:65 Acetonitrile:Water (0.1% TFA)
Flow Rate:	1.0 ml/min
Detection:	254nm
Injection:	10µl
Temp:	Ambient

Test Results for Acidic Compounds Test
Click on any analyte to view its structure.

Column Name (Listed Alphabetically)	Analytes	Rs EPB/2-HBZ	Plates/Meter	Symmetry	USP Tailing
Cogent HPS C18 Catalog No.: 75018-15R Click Here to View Product Literature	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	7.41	67,213 43,600 71,193 78,900 82,607 82,713	1.26 1.10 0.99 0.96 0.98 0.92	1.17 1.05 0.98 0.97 0.97 0.94
Beta Basic C8	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	5.04	67,687 39,507 64,287 72,087 74,800 78,267	1.43 1.13 1.09 1.09 1.17 0.99	1.25 1.11 1.05 1.05 1.11 0.99
Hypersil BDS C18	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	5.51	61,580 31,653 57,433 64,633 70,513 77,040	1.44 1.20 1.19 1.21 1.22 1.12	1.25 1.22 1.11 1.13 1.14 1.08
Inertsil ODS--3	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	7.21	37,760 37,133 59,393 68,413 70,280 73,153	1.51 1.15 1.11 1.10 1.06 1.05	1.33 1.10 1.07 1.06 1.02 1.03
Kromasil C18	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	7.37	41,787 33,667 64,907 73,387 76,853 84,760	1.94 1.28 1.28 1.27 1.26 1.17	1.56 1.19 1.17 1.17 1.12 1.10
Luna C18 (2)	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	7.81	57,013 41,947 72,967 84,933 88,320 93,033	1.59 1.21 1.18 1.15 1.19 1.09	1.39 1.14 1.11 1.10 1.11 1.06
Selectosil C18	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	4.98	62,240 34,900 48,447 54,933 50,953 57,833	1.18 1.09 1.00 0.98 0.92 0.91	1.13 1.07 1.01 0.99 0.95 0.94
Spherisorb ODS 2	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	3.40	47,013 35,093 62,747 69,167 11,427 94,560	1.82 1.29 1.49 2.35 7.98 1.10	1.46 1.21 1.32 1.82 4.88 1.07
YMC Basic C8	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	5.23	64,680 40,420 65,060 70,607 76,327 80,600	1.81 1.37 1.33 1.31 1.32 1.24	1.68 1.32 1.24 1.23 1.23 1.19
Zorbax Extend C18	Uracil 4-Hydoxy-BZA Acetylsalicylic A Benzoic Acid 2-Hydroxy BZA Ethyl Paraben	6.47	52,347 28,113 54,013 65,760 70,573 80,787	1.49 1.26 1.18 1.15 1.15 1.04	1.25 1.20 1.10 1.09 1.07 1.02

Chromatograms





Cogent and HPS are trademarks of MicroSolv Technology Corporation, Inertsil ODS-3 is a trademark of GL Sciences, Kromasil is a trademark of Akzo Nobel, Zorbax Extend is a trademark of Agilent Technologies, Inc., Spherisorb ODS2 and YMC Basic are trademarks of Waters Corporation, Beta Basic C8 and Hypersil BDS C18 are trademarks of Thermo Corporation, Selectosil C18 and Luna C18 are trademarks of Phenomenex Corporation.