|
|
Test Parameters
Identification of Peaks: Peaks were confirmed by retention times against individual
standards and on line UV spectra.
Chromatographic Conditions: The HPLC was performed at ambient temperature on an HP
1090 instrument.
Column Key: All columns were selected with 5µm particles, 150x 4.6mm. The columns
used were as follows:
|
Column Description |
Code |
Supplier |
|
Beta Basic™ C8 |
9 |
Thermo Keystone Operations |
|
Cogent HPS™ C18 |
1 |
MicroSolv Technology Corp. |
|
Hypersil BDS™ C18 |
6 |
Thermo Hypersil |
|
Inertsil™ ODS-3 |
4 |
GL Sciences |
|
Kromasil™ C18 |
2 |
Kromasil/Column Engineering |
|
Luna™ C18(2) |
5 |
Phenomenex |
|
Selectosil™ C18 |
8 |
Phenomenex |
|
Spherisorb™ ODS2 |
7 |
Waters Corporation |
|
YMC Basic™ S-5 |
10 |
Waters Corporation |
|
Zorbax Extend™ C18 |
3 |
Agilent Technologies |
|
Codes 1-5 |
Low Metals |
|
Code 6 |
Non Low Metals |
|
Code 7-8 |
Older Technology |
|
Code 9-10 |
C8, Low Metals |
Extra Column Variance: 67µl, or sigma = 8µl
|
|
