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HPLC Application Sheet #128
Market Leader Column Separating Antidepressants
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Method Conditions:
Column: Brand S C18, 150mm x 4.6mm
Type of Hardware: Compression Fittings
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 215nm
Injection: 10µL
Mobile Phase: 80:20: Methanol:KH2PO4, PH 6.0
Test Mixture: Uracil, Nortriptyline, Toluene, Imipramine, Amitryptiline
Peak
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tR
|
k'
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N/M
|
1: Uracil
|
1.51
|
0.00
|
44,880
|
2: Nortriptyline
|
11.22
|
6.43
|
4,533
|
3: Toluene
|
3.71
|
1.46
|
103,753
|
4: Imipramine
|
not eluted
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5: Amitryptiline
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not eluted
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