| |
HPLC Application Sheet #127
Market Leader Column Separating Antidepressants
< Back to the previous page
Method Conditions:
Column: Brand Y (BDS) C18, 150mm x 4.6mm
Type of Hardware: Compression Fittings
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 215nm
Injection: 10µL
Mobile Phase: 80:20: Methanol:KH2PO4, PH 6.0
Test Mixture: Uracil, Nortriptyline, Toluene, Imipramine, Amitryptiline
Peak
|
tR
|
k'
|
N/M
|
1: Uracil
|
1.67
|
0.00
|
47,153
|
2: Nortriptyline
|
3.22
|
0.93
|
47,713
|
3: Toluene
|
3.11
|
0.87
|
41,400
|
4: Imipramine
|
5.04
|
2.02
|
44,600
|
5: Amitryptiline
|
5.89
|
2.53
|
46,160
|
|
|
