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HPLC Application Sheet #125
Market Leader Column Separating Basic Compounds
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Method Conditions:
Column: Brand S (ODS 2) C18, 150mm x 4.6mm
Type of Hardware: Compression Fittings
Flow Rate: 1.0mL/min
Temperature: Ambient
Detection: 254nm
Injection: 10µL
Mobile Phase: 60:40 Methanol:Water
Test Mixture: Uracil/Pyridine/Phenol
Peak
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tR
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k'
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Symmetry
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USP Tailing
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N/M
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1: Uracil
|
1.46
|
0.00
|
1.16
|
1.13
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38,507
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2: Phenol
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2.60
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0.78
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1.10
|
1.24
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62,333
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3: Pyridine
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3.23
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1.20
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2.88
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2.59
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713
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