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Warfarin Tablet Assay
Excellent Peak Shape on a Rugged Column
Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
52:48 Acetonitrile:DI Water + 10mM Sodium Phosphate Monobasic |
| Flow rate |
1.5 mL/min. |
| Peaks |
Warfarin
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| Injection Volume |
10 µL |
| Detection |
UV 280 nm |
| Temperature |
40°C |
Discussion
This method is easy to prepare, use and reproduce. Note the excellent peak shape for this compound. This column and methodology will
give a long life and many reproducible runs.
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Note: Warfarin (also known under the brand names Coumadin, Jantoven, Marevan, and Waran) is an anticoagulant. It is named
after the Wisconsin Alumni Research Foundation, which sponsored its development. It was initially marketed as a pesticide
against rats and mice, and is still popular for this purpose, although more potent poisons such as brodifacoum have since
been developed. A few years after its introduction, warfarin was found to be effective and relatively safe for preventing
thrombosis and embolism (abnormal formation and migration of blood clots) in many disorders. It was approved for use as a
medication in the early 1950s, and has remained popular ever since; warfarin is the most widely prescribed anticoagulant
drug in North America. Despite its effectiveness, treatment with warfarin has several shortcomings. Many commonly used
medications interact with warfarin, and its activity has to be monitored by frequent blood testing for the international
normalized ratio (INR) to ensure an adequate yet safe dose is taken. Warfarin is a synthetic derivative of coumarin, a
chemical found naturally in many plants, notably woodruff (Galium odoratum, Rubiaceae), and at lower levels in licorice,
lavender, and various other species. Warfarin and related coumarins decrease blood coagulation by inhibiting vitamin K
epoxide reductase, an enzyme that recycles oxidated vitamin K to its reduced form after it has participated in the
carboxylation of several blood coagulation proteins, mainly prothrombin and factor VII. For this reason, drugs in this
class are also referred to as vitamin K antagonists.
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