Norgestrel, ETES & Impurity by HPLC

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Ordering Information

HPLC Application Sheet

Norgestrel, ETES & Impurity (4) - Cogent UDC-Cholesterol™

Chromatogram A
Chromatogram B
Chromatogram C

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Method Conditions

Mobile Phase

	Chromatogram A:	35% Aqueous (0.1%TFA) 65% MeOH
	Chromatogram B:	42% Aqueous (0.1%TFA) 58% MeOH
	Chromatogram C:	45% Aqueous (0.1%TFA) 55% MeOH

Cogent UDC-Cholesterol™

Catalog No.

Temperature

Discussion of Chromatograms

Finding trace impurities is a key element of pharmaceutical research. No trace impurities were found in ETES when using:

both ACN and MeOH with a variety of C18 columns
only ACN at various percentages on a UDC-Cholesterol™ column
MeOH on the UDC-Cholesterol™ column at temperatures above 15°C

At 0°C on the UDC-Cholesterol™ column as the percentage of MeOH is sequentially reduced from 65% to 55%, it becomes obvious that a previously unresolved component is present.
Implications, Advantages & Uses
Using the unique, shape recognition, selectivity mechanism (MeOH) on the UDC-Cholesterol™ column at low temperatures allowed resolution of a previously undetected impurity in a test steroid.