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Sucrose Analysis by LCMS
Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
A: 80% DI water/20% methanol/0.1% formic acid/100 microM sodium acetate
B: 100% acetonitrile+0.2% acetic acid
ATTENTION: Na concentration is in microM. Higher concentration is harmful for MS. |
| Gradient |
| Time (min) | %B | Flow Rate(mL/min) |
| 0.00 | 100 | 0.600 |
| 1.00 | 100 | 0.600 |
| 4.00 | 50 | 0.600 |
| 7.00 | 50 | 0.600 |
| 8.00 | 100 | 0.600 |
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| Post time |
Total gradient time 10 minutes. Post time 5 min |
| Flow rate |
0.4 mL/min. |
| Samples |
Sucrose 10 ppm, m/z 365.1054 (M+Na)+ |
| Detection |
ESI – pos - Agilent 6210 MSD TOF mass spectrometer. |
Discussion
A fast, reliable method of detection and analysis of sucrose was developed
using a Cogent Diamond Hydride HPLC column in the Aqueous Normal Phase (ANP)
mode. The sucrose peak was symmetrical and easy to integrate and the method
is reproducible. This LC-MS method can be used easily and reliably as a
screening test for intestinal disorders in place of the currently used more
cumbersome methods.
Notes: Colon cancer is associated with high intake of sucrose [1]. Determination of
sucrose urinary excretion in absorption test provides information on malnutrition and
has clinical application as screening procedure for gastrointestinal diseases (mainly
celiac disease). Many techniques have been used to quantify sucrose in urine and blood:
thin layer chromatography, colorimetric/enzymatic procedures, HPLC and GC. Many of them
require sample derivatization either for detection or analysis purposes.
[1]. “Sucrose, glucose and fructose have similar genotoxicity in the rat colon and affect
the metabolome”, M. Hansen, D. Baunsgaard, H. Autrup, U.B. Vogel, P. Moller, R.
Lindecrona, H.Wallin, H.E. Poulsen, S. Loft, L.O. Dragsted, Food and Chemical Toxicology
46 (2008) 752-760.
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