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Orthogonal Methods for Propantheline Bromide
Simple methods withouth use of ion-pairing agents
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printable Application Sheet
Notes:
Propantheline bromide is a muscarinic acetylcholine receptor antagonist which is used for the treatment of ulcers, cramps and spasms of the
digestive system, and hyperhidrosis.
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Method Conditions
| Column |
Fig. A: Phenyl Hydride™, 4µm, 100A
Fig. B: Diamond Hydride™, 4µm, 100A |
| Catalog No. |
Fig. A: 69020-7.5P Fig. B: 70000-7.5P |
| Dimensions |
4.6 x 75 mm |
| Solvents |
Fig. A: DI water/ 0.1% formic acid
& nbsp; Fig. B - 97% Acetonitrile/ 3% DI water/ 0.1% formic acid |
| Gradient |
| Fig. A |
Fig. B |
| time (min.) |
%B |
time (min.) |
%B |
| 0 |
10 |
0 |
80 |
| 4 |
50 |
4 |
70 |
| 5 |
10 |
5 |
80 |
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| Flow rate |
1.0 mL/min |
| Injection Volume |
10µL |
| Temperature |
Fig. A: 35° C Fig. B: ambient25° C |
| Sample |
Stock Solution: 1.0 mg propantheline bromide diluted with 1 mL of 50:50 A:B. Working Solution: 100 µL stock diluted with 900
µL 50:50 solvent A: solvent B. |
| Peaks |
Propantheline bromide |
| Detection |
UV 254 nm |
Discussion
In the USP assay method for propantheline bromide tablets, sodium dodecyl sulfate (SDS) is used as an ion-pairing agent in the mobile phase. The long
alkyl chain of this additive makes it particularly difficult and time-consuming to load and remove from the HPLC column. Using CogentTM HPLC columns,
not only is the need for ionpairing agents eliminated but the assay can be performed in either the reversed phase (Phenyl Hydride, Figure A) or aqueous
normal phase mode (Diamond Hydride, Figure B). The mobile phase solvents can be used for many methods using the TYPE-C™ columns, which saves a
laboratory both time and money by not having to prepare specialized mobile phases for every analysis. Both methods illustrate good repeatability, with
each run from the five-run overlays shown in a different color.
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