Quantitation of Prednisone
Simple and Easy by LCMS
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printable Application Sheet
Note: The administration of prednisone, a synthetic analog of cortisone, suppresses production of cortisol and monitoring the concentration of
this corticosteroid has significant therapeutic and clinical importance. Prednisone and prednisolone were introduced by Schering Corporation in
the mid-1960s under the brand names Meticorten and Meticortelone, respectively. These prescription medicines are now available from a number
of manufacturers as generic drugs.
||Cogent Bidentate C18, 4µm, 100A
||2.1 x 50 mm
||A: DI water + 0.1% formic acid
B: methanol + 0.1% formic acid
|Gradient 1||Gradent 2|
|time (min.)||%B ||time (min.)||%B|
||Prednisone 359.1853 m/z (M+H)+
(100 ng/mL prepared in 100%B and diluted 1:10 before analysis.)
||APCI – POS - Agilent 6210 MSD TOF mass spectrometer
The LC/MS method in this note does not need any type of derivatization and has therefore offers a great advantage over other HPLC methods. Two types
are gradients are presented which will allow you to choose one depending on other components in the sample and your method objectives. Retention can
be achieved on a very short column (50 mm) as shown.
Linear gradient conditions were used for analysis of this important corticosteroid. All TYPE-C Cogent columns equilibrate very rapidly to initial
conditions when gradient analysis is used.