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Polyphenols in Grape Seed Extract
Gallic acid, Catechin, and Epicatechin
| Figures: |
A: TIC of grape seed extract
B: Grape seed extract
C: Catechin standard
D: 5 overlaid injections of grape seed extract |
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printable Application Sheet
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Note: Grape seed extracts have a high content of phenolic compounds, such as gallic acid, catechin, epicatechin and procyanidins. They have
been the matter of intense investigations with respect to their potentially beneficial effects on human health. Phenolic compounds are
secondary plant metabolites and they play an important role in plant growth, reproduction, and protection against pathogens and predators.
This data was presented at the American Society of Pharmacognosy Annual Meeting and Exhibition, July 30 – August 3, 2011, San Diego,
California.
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Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-05P-2 |
| Dimensions |
2.1 x 50 mm |
| Solvents |
A: DI water + 0.1% formic acid
B: Methanol + 0.1% formic acid |
| Gradient |
| time (min.) |
%B |
time (min.) |
%B |
| 0 |
10 |
7 |
15 |
| 5 |
15 |
8 |
10 |
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| Injection Volume |
1 microL |
| Detection |
ESI – neg - Agilent 6210 MSD TOF mass spectrometer. |
| Flow rate |
0.4 mL/min |
| Sample |
Grape seed extract tablet was crushed and extracted with DI water at 40°C. Before injection, sample solution was filtered using a
0.45 micron nylon filter (MicroSolv Tech Corp). |
| Peak |
1. Gallic acid 169.0142 m/z (M - H)- ,
RT=0.862 min
2. Catechin 289.0718 m/z (M - H)- ,
RT=3.739 min
3. Epicatechin 289.0718 m/z (M - H)- ,
RT=7.626 min |
| t0 |
0.4 min |
Discussion
The analytical method presented in this study was applied to the determination of phenolics in a grape seed extract. From Figure B, it can be
determined that the aqueous extract contains a high amount of gallic acid, catechin and epicatechin. A Cogent Bidentate C18™ column used for the
analysis was chosen because of rapid equilibration time when a gradient analysis is required.
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