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Retention & Fast Analysis of L(+)-a-phenylglycine for Antibiotic Analysis
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printable Application Sheet
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Note: A chromatogram using a Cogent UDC-Cholesterol column is shown to compare the stronger retention of the Silica-C
75mm column compared with the Cholesterol 150mm column. Both columns used the same mobile phase.
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Retaining the amino acid L(+)-a-phenylglycine using Aqueous Normal Phase Chromatography.
Method Conditions
| Column |
Cogent Silica-C™, 4µm, 100A |
| Catalog No. |
40000-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
80% acetonitrile/19.5% DI water/0.5% formic acid, Pre-mixed |
| Flow rate |
1.0 mL/minute (t0 = 0.89 min) |
| Injection Volume |
5 µL |
| Sample |
L(+)-a-phenylglycine 0.3 mg/mL dissolved in the mobile phase |
| Detection |
UV 254 nm |
Discussion
Cogent Silica-C column produces satisfactory retention of the important amino acid L(+)-a-phenylglycine under isocratic
elution using a simple mobile phase. In addition the retention when using the Cogent Silica-C is extremely reproducible
(%RSD 0.2). Phenylglycine is a synthetic amino acid used in manufacturing lactam antibiotics, such as semi-synthetic
cephalosporines and penicillins. The application above is done using the ANP (aqueous normal phase) mode.
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