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HPLC Application Sheet
Polycyclic Aromatic Hydrocarbons (PAH): A Reverse Phase Separation
Note: Cogent Bidentate C18 with Selectivty3™ operates in three modes of chromatography (reversed phase, aqueous normal
phase and organic normal phase). Separation of PAHs illustrates its excellent capabilities in the reversed phase mode.
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printable Application Sheet
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Method Conditions
| Column |
Cogent Bidentate C18, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75mm |
| Mobile Phase |
Acetonitrile/DI Water 70:30 |
| Flow rate |
0.5 mL/minute |
| Injection Volume |
1 µL |
| Samples |
NaphthalenePhenanthreneFluorenePyreneImpurities or decomposition products - 1mg of
each sample was dissolved in 1 mL of the mobile
phase |
| Detection |
UV at 254 nm |
Discussion
PAH (Polycyclic Aromatic Hydrocarbons) determination in several matrices (soil, food, air, body fluids etc.) is a topic of
interest for routine quality control or screening analyses. Cogent Bidentate C18™ columns offer good resolution,
reproducible retention time and peak shape for PAH compounds. In addition to the main components, small peaks due to
impurities or decomposition products are well resolved.
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