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Monoclonal anti-a1-glycoprotein
From Mouse
Method Conditions
| Column |
Cogent Bidentate C8 300™, 5µm, 300A |
| Catalog No. |
40008-75P-3M |
| Dimensions |
4.6 x 75 mm |
| Solvents |
A: DI water + 0.1% trifluoroacetic acid (TFA)
B: acetonitrile + 0.1% TFA |
| Gradient |
| Time (min.) |
%B |
| 0.0 |
15 |
| 20.0 |
50 |
| 25.0 |
50 |
| 25.1 |
15 |
|
| Post time |
5 min |
| Flow rate |
0.5 mL/min. |
| Sample Peak |
Monoclonal anti-a1-glycoprotein, mouse 043H4848 in 0.05% w/v in DI water
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| Detection |
UV 214 nm. |
| Injection |
1 microL |
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printable Application Sheet
Discussion
For the last few decades glycoproteins have been a subject of interest to biochemists and biologists in many fields. These proteins are
found in plasma and other biological fluids and they serve many functions in nearly every physiological process of living organisms.
These macromolecules consist of a peptide chain and one or more carbohydrates linked to them. The simple gradient method used in this
note for the analysis is very reproducible (%RSD about 1.5). The equilibration time between samples is very short. The peak is
symmetrical and easy to integrate.
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