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HPLC Application Sheet
Metformin & Glyburide - Cogent UDC-Cholesterol™

PEAKS
  1. Metformin
  2. Glyburide
  Method Conditions

Column Cogent UDC-Cholesterol™
Catalog No. 69069-7.5
Dimensions 75mm x 4.6mm ID
Mobile Phase 50% A: Water with 0.05% H3PO4 (pH 2.18)
50% B: Acetonitrile with 0.05% H3PO4
Detection UV, 210nm
Flow Rate 1ml/minute
Back Pressure 600 psi

PDF
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printable Application Sheet


View stationary phase specifications
View chemical structures of analytes
Discussion of the Chromatogram:
Metformin is a highly polar compound that does not usually retain in reverse phase separations. Glyburide is a relatively non polar compound. Getting the two to separate on the same column is very unique. It should be noted that with changes in the percentages of the mobile phase, the peaks for these two anti-diabetic compounds can be made to SWAP elution order or to co-elute them.


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