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Rapid & Simple Analysis of Meprobamate
One Step Sample Prep Plasma or Urine
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printable Application Sheet
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Note: Meprobamate is a carbamate compound used as a drug since the 1950’s. Still widely prescribed as a sedative and more recently in the
treatment of alcoholic withdrawal syndrome, it can be toxic which under some circumstances can lead to death. It is very often involved in
accidental or suicidal overdoses. For all these reasons identification and quantification of this drug is very important in toxicology for
patient monitoring and clinical studies.
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Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-05P-2 |
| Dimensions |
2.1 x 50 mm |
| Mobile Phase |
A: 80% DI water + 0.1 formic acid
B: 20% acetonitrile + 0.1% formic acid |
| Injection |
1 microL |
| Flow Rate |
0.4 mL/min.
t0 = 0.40 min |
| Temperature |
30°C |
| Sample |
Meprobamate 219.1339 m/z (M+H)+
RT = 1.996 min |
| Chromatograms |
A: Standard
B: 1st and 10th injection overlaid |
| Detection |
ESI-POS - Agilent 6210 MSD TOF mass spectrometer |
Discussion
Several methods have been reported in the literature for the quantification of meprobamate in biological samples (plasma, urine, hair etc) for patient
monitoring and other tests. Most of these methods present the drawbacks of long analysis time and complex sample pretreatment (derivatization, solid or
liquid phase extraction).
The analytical strategy presented in this application note is rapid and simple and can be used for quantification in urine or plasma samples. A simple
one-step protein precipitation with acetonitrile is all that is required. When spiked urine samples were analyzed, no significant matrix effect was
observed. Also there was no interference peak in blank urine samples (data not shown). The [13C-2H3]-meprobamate is recommended to be used as an
internal standard and when a lower detection limit is required for the analysis, the use of LC-MS/MS is suggested.
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