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Histamine Retention
LC-MS Compatible Method
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printable Application Sheet
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Note: Histamine is known for its role in allergic response. Release of histamine plays a role in inflammation, gastric acid
secretion, microcirculation and neurotransmission in mammalian brains. Measurement of histamine levels in body fluids has been used in
clinical analysis in various diseases such as pre-eclampsia, asthma, cancer, mastocytosis and in the progression of periodontitis.
Histamine is also present in many foods and beverages, especially red wine and spoiled food. Ingesting histamine can cause migraines,
sweating and nausea.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-7.5P |
| Dimensions |
4.6 x 75 mm |
| Solvents |
| A: | DI H2O/ 0.1% formic acid |
| B: | Acetonitrile/ 0.1% formic acid |
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| Gradient |
| time (min.) |
%B |
| 0 |
80 |
| 5 |
30 |
| 7 |
30 |
| 8 |
80 |
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| Post Time |
5 min |
| Injection Vol. |
1 microL |
| Flow rate |
0.5 mL/min. |
| Detection |
UV 220 nm |
| Sample |
Stock Solution: 1 mg/mL in 80/20 DI H2O/ methanol diluent. The solution was filtered through a 0.45 µm nylon syringe
filter, AQ™ brand (MicroSolv Tech Corp).
Working Solution: Stock solution was diluted 1:10 with 50/50 solvent A/solvent B mixture. |
| Peak |
Histamine |
| t0 |
0.9 min |
Discussion
As a heterocyclic primary amine, histamine is a very polar compound and is difficult to retain on a reverse phase column. The Cogent Diamond
Hydride™ column provides adequate retention and a symmetrical peak shape for this challenging compound without the use of ion pairing
reagents in the mobile phase. The method can be readily transferred to MS detector since the eluents used are MS compatible.
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