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Ultra High Flow Rate, High Efficiency & Selectivity
Standard HPLC Equipment
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printable Application Sheet
Notes:
A) Glucose and sorbitol on Diamond Hydride™ column (2.1 x 150 mm, particle size 4 µm)
B) Comparison of commercial HILIC (4.6 x 150 mm, particle size 3.5 µm) and Diamond
Hydride™ column (4.6 x 150 mm, particle size 4.0 µm) in Aqueous Normal Phase retention
of uracil.
Charts are adapted from “Analysis of Hydrophilic Metabolites in Physiological Fluids by HPLC-MS using a
Silica Hydride-Based Stationary Phase”. J.J. Pesek, M.T. Matyska, J.A. Loo, S.M. Fischer, T.R. Sana, J.
Sep. Sci., 32 (2009) 2200-2208
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
All |
| Solvents |
A: DI water + 0.1% formic acid
B: Acetonitrile+ 0.1% formic acid |
| Mobile Phase |
20% A / 80% B |
| Flow rate |
Variable |
| Injection Volume |
10 microL |
| Detection |
UV (Uracil) and LC-MS (Sorbitol, Glucose) |
| Figures |
Plots of efficiency (HETP) vs. flow rate |
Discussion
Figure A shows a plot of “Flow Rate vs. HETP” for two carbohydrates analyzed using a 2.1 x 150 mm column.
From the plot it is evident that while the flow rate is increased by a factor of 2.5 the efficiency is only
slightly lower (~15%). When a 1 mm column was used over the same flow rates (linear flow rates more than four
times higher) the decrease in efficiency was only 20%.
Figure B shows a plot of “Efficiency vs. Flow Rate” for uracil using a 4.6 x 150mm column. From the plot
it can be seen that efficiency increases as the flow rate increases from 0.3 to 1.0 mL/min. In contrast when a
market leading HILIC column was used under the same conditions, the efficiency dropped by more than 30% over
this same range of flow rates. It should be noted that linear flow rates are more than 6 times greater in the
2.1 mm column than in the 4.6 mm.
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