|
|
Separating Related Compounds
Gentian Violet and Degradation Products
Click here to view
printable Application Sheet
| Note: Gentian Violet is an antifungal agent, staining agent (gram stain test), topical ointment for burns and for finger
printing. This product does not require a prescription but is not easily found in most drug stores. Typically it is prepared
as a weak solution (0.1%) in water and is painted on skin and gums to fight off fungal infections. Gentian Violet has many
uses and is also known as Andergon, Anailine violet, Brilliant Violet 58, Meroxylan, Methyl Violet 10BNS, Vianin and others.
Gentian Violet refers to is color and is not made from gentians. |
|
Method Conditions
| Column |
Cogent HPS Cyano™, 5µm, 120A |
| Catalog No. |
75025-15P |
| Dimensions |
4.6 x 150mm |
| Mobile Phase |
| Solvents: |
A: 10% Acetonitrile+90% 10mM NH4H2PO4
B: 70% Acetonitrile+30% 10mM NH4H2PO4 |
| Gradient: |
0-100% B over 15 minutes (linear gradient) |
|
| Flow rate |
1.5 mL/minute |
| Peaks |
1-3. Degradation Products
4. Gentian Violet |
| Injection Volume |
20 µL |
| Detection |
UV 588 nm |
| Temperature |
25°C |
| Solubility |
Water & Chloroform. Insoluble in ether. |
Discussion
Gentian Violet degrades in solution, forming complex set of degradation products. This method can easily separate out the various
compounds formed in solution. The figure on the left shows a USP standard of Gentian Violet after stressing it in solution thus
creating degradation products. With excellent selectivity as shown as well as great peak shape this method is robust and easy to
perform.
The HPS Cyano column is very stable and produces very high efficiency in this method making it a great choice for analysis.
|
