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Stable Method and Column with Ion Pair Reagents
Guanfacine HCl and Degradants
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printable Application Sheet
| Note: Guanfacine can be used to control high blood pressure by reducing the heart rate and relaxing blood vessels but also is
prescribed for ADD and ADHD and sometimes for post-traumatic stress syndrome. Guanfacine has been known to reduce nightmares
and flashbacks. |
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Method Conditions
| Column |
Cogent Bidentate C8™, 4µm, 100A |
| Catalog No. |
40008-15P |
| Dimensions |
4.6 x 150mm |
| Mobile Phase |
30% Acetonitrile, 70% DI Water w/Conc Phosphoric Acid (1mL/L), 1 g/L SDS |
| Flow rate |
1.5 mL/minute |
| Peaks |
1. Guanfacine
2. Primary Degradant 1
3. Primary Degradant 2 |
| Injection Volume |
20 µL |
| Detection |
UV 220 nm |
| Temperature |
25°C |
Discussion
The US Pharmacopeia lists several known degradation products for Guanfacine. This figure shows a separation of a degraded Guanfacine
drug substance. This method produces very efficient results but what is important to note is that this column is extremely stable
under the very aggressive mobile phase conditions needed. Using columns based on silica hydride instead of ordinary silica offers not
only stability but great compatibility with SDS (sodium dodecyl sulfate) and other ion pair reagents.
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