|
|
Furosemide USP & Related Compound
Diuretic Drug: Furosemide Tablet Formulation

Click here to view printable Application Sheet
|
Note: Furosemide or frusemide is a loop diuretic used in the treatment of congestive heart failure and edema. It is most
commonly marketed by Sanofi-Aventis under the brand name Lasix. It has also been used to prevent thoroughbred and standard
bred race horses from bleeding through the nose during races. Along with some other diuretics, Furosemide is also included on
the World Anti-Doping Agency's banned drug list due to its alleged use as a masking agent for other drugs.
|
|
Method Conditions
| Column |
Cogent Bidentate C18, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile Phase |
70% Water, 30% THF 1% Acetic Acid |
| Flow rate |
1.0 mL/minute |
| Peaks |
1. Furosemide
2. Related compound
|
| Injection Volume |
20 µL |
| Detection |
UV 254 nm |
| Temperature |
25°C |
Discussion
This active pharmaceutical ingredient, Furosemide USP and its related compound can be a difficult molecule to chromatograph with
conventional L1 (C18) columns due to silanol activity.
Note the excellent peak shape of Furosemide and its related compound A when used with this method; baseline resolution is achieved
between this specified impurity and Furosemide. The active is easily separated from excipients in this tablet formulation.
MW of the Furosemide is 330.75.
|