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A Column Comparison In A Sensitive Method For
EDTA: ethylenediaminetetraacetic acid
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printable Application Sheet
| Note: EDTA is a synthetic metal complexing reagent that is used in a wide variety of industrial applications. Used as a
preservative, it has very low biodegradability thus remains in the environment for long periods of time. Found in sewer
water, freshwater and ground water, it re-solubilizes precipitated toxic metals back into solution where they can be ingested
by plants and animals. |
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Method Conditions
| Column 1 |
Cogent HPS C8™, 5µm, 120A |
| Column 2 |
Waters® Symmetry C8™ |
| Catalog No. |
75008-15P |
| Dimensions |
4.6 x 150mm |
| Mobile Phase |
2% Acetonitrile, 98% DI Water 0.1% Acetic Acid
pH 3.5, plus 2g/L Tetrabutylammonium sulfate |
| Flow rate |
2.0 mL/minute |
| Peaks |
1. Water (solvent front)
2. EDTA Fe3+ |
| Injection Volume |
20 µL |
| Detection |
UV 258 nm |
| Temperature |
40°C |
| LOQ |
0.2µg/mL |
Discussion
Detectable using UV detectors and it also helps in the selectivity of the compound. This method produces excellent peak shapes and
selectivity using the Cogent HPS C8™ making it a great choice. Comparing the Cogent HPS C8™ to a market leader, Waters
Symmetry C8™, you can see that the columns are equal in every chromatography aspect.
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