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Domoic Acid
Determination of domoic acid (DA) without derivatization
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printable Application Sheet
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Notes: Domoic acid, a compound known for over 50 years, was found to have neurological effects when ingested by humans. It is the chemical
responsible for amnesiac shellfish poisoning (ASP). Upon ingestion by humans, it can cause diarrhea, dizziness, seizures, permanent loss of
short-term memory, and even death. In Canada, the European Union, and the USA, the legal limit is 20 microg DA/g in wet tissues of shellfish.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-05P-2 |
| Dimensions |
2.1 x 50 mm |
| Solvents |
| A: | 50% methanol/ 50% DI water/ 0.1% formic acid |
| B: | 97% Acetonitrile/ 3% DI water/ 0.1% formic acid |
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| Gradient |
| time (min.) |
%B |
time (min.) |
%B |
| 0 |
95 |
11 |
30 |
| 10 |
30 |
12 |
95 |
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| Post Time |
5 min |
| Injection Vol. |
1 microL |
| Flow rate |
0.4 mL/min. |
| Detection |
ESI - POS - Agilent 6210 MSD TOF mass spectrometer |
| Sample |
Stock Solution: 1 mg/mL DA in methanol diluent.
Working solution: Stock was diluted using 50% solvent A and 50% solvent B mixture for the final concentration 0.5 mg/L. Before
injection, solution was filtered using a 0.45 micron nylon filter (MicroSolv Tech Corp). |
| Peak |
Domoic acid 312.1442 m/z (M+H)+ |
Discussion
To date, several analytical methods have been developed for the quantitative determination of domoic acid (DA). However, many of the methods require
tedious and labor-intensive derivatization of this compound in order to detect it. A new method based on HPLC and MS detection which does not require
derivatization has been developed. A simple linear gradient and typical MS compatible mobile phases were used to retain DA, a potent neurotoxin. The
advantage of using Cogent columns is that they equilibrate rapidly when a gradient is used. The method can be used by regulatory agencies responsible
for monitoring the occurrence of toxins for analysis of domoic acid in seafood samples and other laboratories.
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