|
|
Fast & Preceise Bisphenol A (BPA) Method
Toxic Substance Found in Consumer Products
Click here to view
printable Application Sheet
|
Note: BPA is a synthetic compound widely used in industry as an epoxy resin, a polycarbonate, and an antioxidant in polyvinyl chloride
(PVC) plastics. Epoxy resins are used as inner surface coatings for food and beverage cans. Polycarbonates are used in fabrication of
plastic food containers (even infant feeding bottles). PVC is in a variety of products which come in contact with food. Even at low
concentrations, chronic exposure to BPA is of toxicological concern. It has estrogen-like effects and causes a variety of adverse
symptoms (e.g. genital malformations, testicular abnormalities, impairment in fertility or sexual functions).
|
|
Method Conditions
| Column |
Cogent Bidentate C8™, 4µm, 100A |
| Catalog No. |
40008-75P |
| Dimensions |
4.6 x 75 mm |
| Mobile phase |
A: DI H2O/0.1% formic acid
B: Acetonitrile/0.1% formic acid |
| Gradient |
| Time (min) |
%B |
| 0 |
30 |
| 2 |
30 |
| 6 |
90 |
| 8 |
90 |
| 9 |
30 |
|
| Flow rate |
0.5 mL/min |
| Peaks |
Bisphenol A |
| Injection Volume |
5 µL
|
| Detection |
UV 275 nm |
| t0 |
0.9 min |
Discussion
Bisphenol A (BPA) is a challenging compound for analysis by HPLC. Biological monitoring of this environmental and health toxicant is a necessary
process for surveillance as well as risk assessment. As can be seen from the accompanying chromatograms, a Cogent Bidentate C8™ column was
an excellent choice for the analysis of BPA. The peak shape is symmetrical with high efficiency.
The repeatability of the analysis is also
remarkable as can be seen in the figure, which shows five overlaid injections of the compound. In addition, the method equilibrates rapidly with
only 1 minute post time after the gradient.
|
