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HPLC Application Sheet
Biological Fluids: Choline & Acetylcholine
Method Conditions
| Column |
Cogent Qx2™ Column for LC-MS UDC-Cholesterol, 4µm, 100A |
| Catalog No. |
69069-Q20 |
| Dimensions |
2.1 x 20 mm |
| Mobile Phase |
91:9 acetonitrile/DI water + 0.5% formic acid
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| Flow rate |
0.3 mL/minute |
| Injection Volume |
1 µL |
| Samples |
Acetylcholine (m/z 146)
Choline (m/z 104)
(5 pmol of each injected on column) |
| Detection |
Mass Spec: Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring |
Discussion
The "Aqueous Normal Phase" experimental condiions shown above allow for effective separation of small polar compounds
on a moderately hydrophobic stationary phase. The short and fast Qx2 format of these columns adds advantages of
significantly reduced analysis time when compared with a standard column (see MicroSolv Application Note B05-1). Mass
detection provides for analysis at biologically and environmentally relevant concentration levels.
Note: the model compounds used in the study are very difficult to retain on traditional hydrophobic columns.
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