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HPLC Application Sheet
B-Vitamins - Cogent Bidentate C18™

Method Conditions

Column Cogent Bidentate C18™
Catalog No. 40018-25
Dimensions 250mm x 4.6mm
Mobile Phase   
  0-4 minutes: 5% Acetonitrile: 95% Water/TFA
    (0.1% v/v TFA DI Water)
4-10 minutes: 15% Acetonitrile: 85% Water/TFA
    (0.1% v/v TFA DI Water)
10-15 minutes: 5% Acetonitrile: 95% Water/TFA
    (0.1% v/v TFA DI Water)
Flow Rate 1ml/minute
Wavelength 270nm
Injection Volume 5ul


PEAKS

  1. Niacinamide: CAS No. 98-92-0 (3-Pyridinecarboxamide)
  2. Thiamine (mono-nitrate): CAS No. 532-43-4
  3. Pyriadoxine HCL: CAS No. 65-23-6
  4. Folic Acid: CAS No. 59-30-3
  5. Riboflavin: CAS No. 83-88-5
View stationary phase specifications
View chemical structures of analytes

PDF
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printable Application Sheet

Discussion of the Chromatogram:
Standard C18 columns will not be retentive enough to retain these very polar compounds. The Cogent Bidentate C18™ will retain them and due to the chemical nature of the TYPE-C Silica™, the column quickly equilibrates between mobile phase changes making this separation possible with a single pump.


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