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HPLC Application Sheet
Bidentate C8™ column for more Hydrophobic compounds

Shorter Analysis Time

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TABLE 1
Column k1'
tert.-butylbenzene		 k2'
anthracene		 a=k2'/k1'
A. Cogent C8 4.19 4.57 1.10
B. Cogent BDC18 19.98 31.49 1.58


Method Conditions


Column A. Cogent Bidentate C8 (Octyl), 4µm, 100A
B. Cogent Bidentate C18 (ODS), 4µm, 100A
Catalog No. A. 40008-75P
B. 40018-7.5P
Dimensions 4.6 x 75mm
Mobile Phase 70:30 methanol/DI water
Flow rate 1.0 mL/minute
Injection Volume  5 µL
Sample Universal LC Test Mix
   1. benzene
   2. toluene
   3. ethylbenzene
   4. isopropyl benzene
   5. tert.-butylbenzene
   6. anthracene
Detection UV 254 nm


Discussion

For separations requiring a less hydrophobic stationary phase Cogent C8 can be used successfully as demonstrated in the chromatograms. The retention on the C8 column is lower when compared with a C18 column under the same mobile phase conditions, but since the efficiency is excellent the separation goals can be achieved in a shorter time. The C8 phase is suited for more hydrophobic proteins and peptides and large nonpolar organic compounds.

For increased retention higher percentages of water in the mobile phase can be used. The diminished hydrophobicity is shown in Table 1.






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