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HPLC Application Sheet
Organic Acids - Cogent Bidentate C18™

Method Conditions

Column Cogent Bidentate C18™
Catalog No. 40018-7.5R
Dimensions 75mm x 4.6mm id
Flow Rate 1ml/minute
Mobile Phase 100% Aqueous H3PO4
0.05% v/v
Detection UV 255nm

PEAKS
  1. Oxalic Acid
  2. Formic Acid
  3. Acetic Acid
  4. Sodium Azide
  5. Uracil
  6. Fumaric Acid
  7. Propionic Acid

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View stationary phase specifications
View chemical structures of analytes
Discussion of the Chromatogram:
These very polar compounds are very difficult to hold up in reverse phase on traditional C18 HPLC Columns. The Cogent Bidentate C18™ is able to retain and separate these compounds isocratically in 100% Aqueous condition without the loss of retention time, efficiency or peak shape. In this experiment this column is run for 3 hours with exposure to Aqueous conditions with no change in the results.


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