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HPLC Application Sheet
Organic Acids (3) - Cogent Bidentate C18™, Rapid Development
Discussion of Chromatograms Eight columns with different selectivities were chosen for this study. Both of the type-B, C18 columns exhibited retention time shifts with prolonged exposure to 100% Aqueous conditions. None of the other type-B phases resolved the mixture. Chromatogram A: The Cogent UDC Cholesterol™ column does not show retention shifts with prolonged exposure to 100% Aqueous, but its resolution was not optimal for this sample. This column’s unique selectivity is best suited to compounds that contain ring structures. Chromatogram B: The Cogent Bidentate C18™ column produced stability and optimal resolution when used for prolonged periods with 100% Aqueous. Implications Using 8 (short) columns with different selectivities allowed for the quick choice of the optimal phase – the Bidentate C18™. Advantages & Uses Since columns that are 75x4.6mm can be equilibrated and initially run at 3 ml/min, then at 1 ml/min for final selection, non-suitable phase selectivities can be quickly determined. Once the optimal phase is selected, the appropriate solvent system can be determined... Rapid Method Development. View stationary phase specifications View chemical structures of analytes |
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