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HPLC Application Sheet
Loratadine (2) - Cogent Bidentate C18™,
Rapid Development
Generic Method Conditions
| Mobile Phase |
A: Aqueous (0.1%TFA)
B: THF
C: Hexane |
| Column |
Cogent Bidentate-C18™ |
| Cat. No. |
40018-7.5R |
| Dimensions |
75mm x 4.6mm ID |
| Flow Rate |
1.0ml/mn |
| Detection |
UV, 255nm |
View stationary phase specifications
View chemical structures of analytes
Discussion of Chromatograms
Chromatogram A RP- Reverse Phase: Loratadine in 100% THF elutes with the solvent front. With 70% Aqueous/
30% THF, the selectivity for this compound is classic reverse phase; based on polarity.
Chromatogram B ONP – Normal Phase: At 75% Hexane/ 25% THF on this Cogent Bidentate-C18™ column, the
selectivity for Loratadine is based on functionality.
Implications
Different selectivity based on polarity or functionality can be chosen with the same HPLC column (Cogent
Bidentate-C18™).
Advantages
Cogent Bidentate-C18™ columns do not suffer from the normal difficulties experienced with traditional HPLC
Columns such as Cyano, Pentafluorophenyl (F5) or Amino, when converting from Aqueous to Organic mobile phases and
back again; continual conversions do not damage or alter the Cogent Bidentate-C18™ columns.
Uses
- Orthogonal Peak Identification.
- Organic Normal Phase separations on a C18 phase to achieve separations that are not possible without using
ion-pair reagents in Reverse Phase.
- Single Column to scout for optimal methods in both Normal and Reverse Phase mode.
- ARP/ANP – Analytical Chromatography.
- ONP- Preparative Chromatography.
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