|
|
HPLC Application Sheet
Using a TYPE-C Column for Routine Reverse Phase: Benzophenone
Click here to view
printable Application Sheet
Note: When the percent of acetonitrile increases (from 70% in A to 80% in B) then the retention time of benzophenone
(hydrophobic non ionic compound) decreases.
|
Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-75P |
| Dimensions |
4.6 x 75mm |
| Mobile Phase |
A: 70% acetonitrile/30% DI water
B: 80% acetonitrile/20% DI water |
| Flow rate |
0.5 mL/minute |
| Injection Volume |
1 µL |
| Sample |
Benzophenone
0.1 mg in the mobile phase
|
| Detection |
UV 254 nm |
Discussion
Benzophenone, a hydrophobic non ionic compound, was selected to illustrate the excellent RP-HPLC properties of Cogent TYPE-C
bonded phases. The results achieved regarding reproducibility, efficiency and peak symmetry were excellent. Each figure
represents ten consecutive injections.
|
