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Anatoxin-a (ANTX-A)
Potent Neurotoxin
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printable Application Sheet
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Notes: Anatoxin-a (ANTX-A) is a cyanobacterial neurotoxin, implicated in many animal and human poisoning incidents. ANTX-A blocks
neurotransmission causing death by respiratory arrest. The presence of this toxin in freshwater has to be monitored in order to prevent
fatalities.
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Method Conditions
| Column |
Cogent Diamond Hydride™, 4µm, 100A |
| Catalog No. |
70000-15P-2 |
| Dimensions |
2.1 x 150 mm |
| Solvents |
| A: | 50% MeOH/ 50% DI H2O/ 0.1% formic acid |
| B: | Acetonitrile/ 0.1% formic acid |
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| Gradient |
| time (min.) |
%B |
time (min.) |
%B |
| 0 |
70 |
6 |
30 |
| 5 |
30 |
7 |
70 |
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| Post Time |
5 min |
| Injection Vol. |
1 microL |
| Flow rate |
0.4 mL/min. |
| Temperature |
25°C |
| Detection |
ESI - POS - Agilent 6210 MSD TOF MS |
| Samples |
Stock standard solution: (0.5 mg/mL) of Anatoxin-a fumarate was prepared in DI water and stored at -20 °C.
Working solution: Sample for injection was diluted 1:100 using 50%A/50%B solvent mixture. It was stored in the dark at 4 °C. |
| Peak |
Anatoxin-a, 166.1226 m/z (M + H)+ |
| t0 |
0.9 min |
Discussion
Anatoxin-a is a low molecular weight, secondary amine and can be difficult to retain with reversed-phase columns. This application note
illustrates a very simple method for retention of Anatoxin-a which does not require derivatization. The retention achieved is good, and the
excellent repeatability is demonstrated by the overlay of five injections shown in the figure. If desired, a lower retention time of Anatoxin-a
can be obtained by changing the starting concentration of solvent B in the gradient to 60%, designing a steeper gradient, or using a shorter
column (e.g. 2.1 x 50 mm).
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