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Amoxicillin
Robust USP Assay Analysis
Click here to view
printable Application Sheet
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Note: Amoxicillin is a beta-lactam antibiotic used to treat a variety of bacterial infections. Its mechanism of action is by inhibition of the
synthesis of bacterial cell walls.
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Method Conditions
| Column |
Cogent Bidentate C18™, 4µm, 100A |
| Catalog No. |
40018-25P |
| Dimensions |
4.6 x 250 mm |
| Mobile Phase |
3: 97 acetonitrile: dsiluent |
| Diluent |
6.8 g/L monobasic potassium phosphate adjusted to pH 5.0 with 45% (w/w) potassium hydroxide |
| Injection Volume |
10 microL |
| Flow rate |
1.0 mL/min |
| Detection |
UV 230 nm |
| Sample |
Figure A: 1.2 mg/mL amoxicillin trihydrate USP RS in diluent.
Figure B: 0.012 mg/mL amoxicillin trihydrate USP RS in diluent. |
| Peak |
Amoxicillin |
| t0 |
2.3 min |
Discussion
The USP assay method for amoxicillin often yields poor peak shapes and low method reproducibility with L1 columns based on type B silica. In contrast,
the figures shown here illustrate the high degree of reproducibility obtained from use of a Cogent Bidentate C18™ L1 column following the USP
assay method. Each figure shows a five run overlay of consecutive runs. Figure A shows the data obtained using the analyte concentration specified by
the USP assay method. Figure B shows a lower concentration which is less likely to result in column overload.
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