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 White Paper |
What is Aqueous Normal Phase (ANP) and how can it help you?
Aqueous Normal Phase (ANP) is a full mode of HPLC. Reverse Phase (RP) and Normal Phase (NP) HPLC are more well known but ANP offers chromatographers benefits that are not possible with other modes. In Aqueous-Normal Phase, the maximum retention time of target compounds is with 100% organic solvent (least polar solvent). As you increase the polar solvent content (Aqueous), the retention reduces to a minimum when the mobile phase is at 70% organic solvent. You get Normal Phase mode using Aqueous or Reverse Phase Solvents. Polar compounds that would not retain in RP for example, retain very well in ANP. By definition: Normal-Phase Chromatography (NP)is a chromatographic mode that will increase the retention of a target compound as the mobile phase becomes less polar (a decrease in concentration of the most polar solvent, often water) conversely the compound has longest retention in a non-polar solvent such as 100% hexane. The main separation mechanism is based on the functionality of the analytes. Reverse-Phase Chromatography (RP) is the opposite or the reverse of the above; there is an increase in retention time of the target compounds as the mobile phase becomes more polar (increase concentration of the most polar solvent, often water). The main separation mechanisms of RP is based on the hydrophobicity of the analytes. Aqueous Normal-Phase Chromatography (ANP) is defined as a normal phase separation pattern using the reverse phase solvents Water and an organic solvent. The main separation mechanisms of ANP is based on the polarity (hydrophilicity) of the analytes.
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