1. How do the PEG-100 Columns provide excellent results when used with Biomolecules?
2. What effect does the PEG layer have on EOF?
3. What benefit does the cross linking of the PEG in the PEG-100 Column provide to me?
4. Does the PEG-100 increase my EOF and migration time precision?
5. Will my analyte absorb the polymer?
6. What column widths does PEG-100 come in?
7. Can I use the MicroSolvCE Window Maker™ or similar units on the PEG-100?
8. Is the conditioning of the PEG-100 the same as with a bare fused silica capillary?
9. What pH range can I operate the PEG-100 HPCE Columns in?

1. How do the PEG-100 Columns provide excellent results when used with Biomolecules? [top]
We bond to bare fused silica capillaries, a dense layer of Polyethylene Glycol (PEG) on the inside diameter that creates a weakly hydrophilic environment. The PEG coating exhibits a low hydrogen bonding capacity and when your analytes are hydrophobic Biomolecules they show a reduced affinity for the wall.

2. What effect does the PEG layer have on EOF? [top]
This layer on the walls of the capillaries will reduce EOF compared to bare fused silica.

3. What benefit does the cross linking of the PEG in the PEG-100 Column provide to me? [top]
The cross linking will increase the likelihood that all the active silanol sites of the bare fused silica are covered. It is these sites that Biomolecules interact with causing peak tailing or wall adsorption.

4. Does the PEG-100 increase my EOF and migration time precision? [top]
Yes, one of the main reasons for poor results due to variable flow is bonding to silanol sites on the capillary wall. The low hydrogen bonding capacity of the PEG coating reduces this effect to nearly zero for many molecules. Each time a molecule is bonded to an active site on the capillary wall, the EOF immediately changes. The more sites that are changed during a run, the more the EOF will exhibit noticeable change.

5. Will my analyte absorb the polymer? [top]
The PEG-100 usually produces a very low sample to polymer interaction and absorption. Your analytes could be different.

6. What column widths does PEG-100 come in? [top]
The PEG-100 is available in both 50µ and 75µ ID width. The length of the column is 1 meter.

7. Can I use the MicroSolvCE Window Maker™ or similar units on the PEG-100? [top]
Yes.

8. Is the conditioning of the PEG-100 the same as with a bare fused silica capillary? [top]
Yes.