| |
CLICK HERE to visit Cogent HPLC Columns Home Page
|
How to Condition a Cogent Column for Storage
|
Long Term Storage:
- Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to
use non-buffered mobile phase.
- Then purge the column with 8 column volumes of 100% HPLC Grade, filtered Isopropyl
Alcohol (IPA).
- Then purge the column with 80% IPA and 20% water with 8 column volumes.
- Cap both ends of the column with the provided protective end caps or with Super-Link (Seal-Links) End Fittings
and capping them with column plugs provided.
- Return column to the box it came in for safekeeping.
- Store column at room temperature.
- Never let the column dry out.
- Never store the column in buffers.
- Never store the column in liquid that is more than 30% water.
Overnight:
- Rinse the column with 8 column volumes with pure solvent that you use as your mobile phase. It is important to
use non-buffered mobile phase.
- Cap both ends of the column with the provided protective end caps or with Super-Link End Fittings and capping
them with column plugs provided.
|
|
