Vitamin B1 & B6

• Low pH yet High EOF
• Short Analysis Times
• Simple, Easy, Fast

Method Conditions: Voltage: 30 kV Capillary: 50µm x 60cm Bare Fused Silica Injection: e-gram 1: 5 seconds hydrodynamic e-gram 2: 30 seconds Run Buffer: CElixir™ Accelerator Solution B, pH 2.5 Detection: UV, e-gram 1: 200nm e-gram 2: 190nm

Method Discussion:
Using a Beckman MDQ CE instrument and a MicroSolvCE bare fused silica capillary, made by Polymicro Technologies, the separation of vitamins B1 and B6 was accomplished in less than 6.0 minutes. In electrophergram one, the sample concentration was 6µg/ml with the peaks being well resolved. In electropherogram 2, the concentration was only 0.5µg/ml and yet two resolved peaks could be detected. The UV wavelength was changed to 190nm to maximize sensitivity and the run buffer was added to the sample.

It is good to note that the migration times of both peaks remain relatively constant and fast in both the separation methods.