MES-Histadine Buffer – pH 6.00
(0.3M MES, 0.21 M His)

Preparation of 100mL of buffer pH 6.00 for use with CelerityCE™ bonded capillaries.

Dissolve 5.8 g of reagent grade MES (FW:195.2) + 3.26 g of reagent grade Histidine (FW 155.2) in 100 ml of de-ionized water. Using every precaution, adjust pH as needed. Keep refrigerated. Dilute (1:10) before use.

MES = (2-[N-Morpholino]ethanesulfonic acid)
HIS = Histidine